InCELL Hunter™ A549 EP300 Bromodomain Cell Line

The InCELL Hunter™ technology measures compound binding to an intracellular target by detecting changes in protein stability. In this assay, the target is fused to the enhanced ProLabel™(ePL) enzyme fragment and expressed in the selected cell background. The amount of fusion is quantified through the addition of substrate and complementary enzyme acceptor fragment (EA). The small size, but robust activity of the ePL tag makes it an ideal fusion partner. The small disordered peptide has minimal effects on target half-life, and generates high levels of enzyme activity, resulting in a sensitive measurement of protein levels.

Catalog No. Size Price Quantity
96-0073C15 2 vials $10,299.00

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Related Family Members:

Receptor Family
Accession Number
Prolink™ Tag
Cell Background
  • [User Manual] InCELL Hunter™ Bromodomain Cell-Based Assay

    InCELL Hunter™ Bromodomain Cell-Based Assays, when used in conjunction with a InCELL Hunter Detection Kit (96-0002, 96-0002L or 96-0002XL), provides a cell based assay to look at protein stability upon compound binding. The assay described in this booklet have been validated for use in 384-well microplate formats.

    [User Manual] InCELL Hunter™ Bromodomain Cell-Based Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
96-0079 InCELL Detection Kit 216 mL (16 plates), 1,600/6,400 dp (96-well/384-well) $3,829.00
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $349.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $299.00
93-0563R7A AssayComplete™ Cell Plating 7 Reagent 100 mL $299.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $239.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00