PathHunter® U2OS CD137 Signaling Cell Line

The PathHunter CD137 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of CD137 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged CD137. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA. This stable modified cell line is provided as two vials of cryopreserved cells.

Catalog No. Size Price Quantity
93-1089C3 2 vials $37,039.00

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Receptor Family
Tumor Necrosis Factor Receptor Superfamily Receptor
Accession Number
Cell Background
  • [User Manual] PathHunter® Checkpoint Assay - TNF Receptor Superfamily (TNFRSF)

    The PathHunter Checkpoint Signaling Assay for Tumor Necrosis Factor Receptor Superfamily (TNFRSF) members (or receptors) is a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting the TNFR superfamily of receptors that activate the non-canonical NF-kB pathway through NF-kB inducing kinase (NIK). The assay is a robust measurement of co-s...

    [User Manual] PathHunter® Checkpoint Assay - TNF Receptor Superfamily (TNFRSF)