1321N1 P2RY1 Gq Cell Line

DiscoverX® Calcium cell lines overexpress naturally Gq coupled, wildtype GPCRs and are designed to detect calcium mobilization in response to agonist stimulation of the receptor. Signal can be detected using the Calcium No WashPLUS Detection Kit.

Catalog No. Size Price Quantity
95-1018C6 2 vials $10,299.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
P2Y
Coupling
Gq
Accession Number
NM_002563.2
Species
Human
Prolink™ Tag
None
Cell Background
1321N1
Agonist/Ligand
2-(methylthio)-ATP
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Culture media was removed and replaced with dye loading buffer from the Calcium No WashPLUS detection kit according to the recommended protocol. Target cells and native parental cells were stimulated with a control agonist and calcium mobilization was detected using a FLIPR® Tetra (Molecular Devices, Inc.). Please refer to page 2 for recommended assay reagents, detection reagents, and control compounds.
  • [User Manual] Native Calcium GPCR Assays

    Native Calcium GPCR Assays provide a fast, reliable method for detection of changes in intracellular calcium. Cells expressing a receptor of interest that signals through calcium are pre-loaded with a calcium-sensitive dye and then treated with compound. Upon stimulation, the receptor signals release of intracellular calcium, which results in an increase of dye...

    [User Manual] Native Calcium GPCR Assays

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
92-3105G AssayComplete™ Cell Culture Kit-105 Kit $349.00
90-0091 Calcium No WashPLUS 10 plates (384-well) $299.00
92-5101FR AssayComplete™ Freezing Reagent F1 2 x 50 mL $299.00
93-0563R7A AssayComplete™ Cell Plating 7 Reagent 100 mL $299.00
92-4101TR AssayComplete™ Thawing Reagent T1 100 mL $239.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00