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PathHunter® eXpress VIPR2 CHO-K1 β-Arrestin GPCR Assay

PathHunter® eXpress β-Arrestin GPCR cells are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0317E2CP0S 100 dp (1 x 96-well) $1378.00
93-0317E2CP0M 200 dp (2 x 96-well) $2058.00
93-0317E2CP0L 1000 dp (10 x 96-well) $7155.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Vasoactive intestinal peptide receptor 2
Group
VIP and PACAP
Cell Background
CHO-K1
Species
Human
Accession Number
NM_003382
Agonist/Ligand
VIP, Human
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gs & Gq
  • [User Manual] PathHunter® β-Arrestin eXpress GPCR Assay

    PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e., β-arrestin recruitment to activated GPCRs, enabling scientist...

    [User Manual] PathHunter® β-Arrestin eXpress GPCR Assay