The PathHunter U2OS TrkB-P75 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Recombinant Human b-NGF) based on activation of TrkB-P75 Kinase activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged TrkB-P75 and EA-tagged . Activation of TrkB-P75 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.