PathHunter® U2OS TrkB-P75 Bioassay Kit

PathHunter® Bioassay kits provide a robust, highly reproducible and easy-to-use, cell-based functional assay to study receptor activity through a chemiluminescent output. The bioassay kits contain the reagents needed for a functional assay including ready-to-assay cells, plating, dilution & detection reagents, positive control agonist and assay plates. The ready-to-assay kits are designed to accelerate and simplify development of a QC potency assay or a NAb assay for biologic drugs.

Catalog No. Size Price Quantity
93-0530Y3-00076 1000 dp (10 x 96-well) $7,729.00
93-0530Y3-00075 200 dp (2 x 96-well) $2,249.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Neurotrophic tyrosine kinase receptor type 2
Group
Receptor Tyrosine Kinase
SH2 Domain
SHC1
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_006180
Agonist/Ligand
Recombinant Human BDNF
Assay Measures
SH2 Recruitment
Bioassay Target Name
TrkB-P75
Assay Qualified With
BDNF
Cells expressing human ErbB1 receptor were seeded in Cell Plating 17 Reagent and incubated with increasing concentrations of an anti-ErbB1 MAb, keeping agonist, human EGF, constant at the EC80 according to the protocol. ErbB1 Bioassay Kit is able to detect a dose dependent neutralization of receptor signaling as increasing concentrations of anti-ErbB1 mAb bind directly to the receptor and block EGF ligand signaling.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
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