PathHunter® U2OS TrkA-p75 Bioassay Kit

The PathHunter U2OS TrkA-p75 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. FSH, Human) based on activation of TrkA-p75 Kinase activity via detection of . The included cell line overexpresses ProLink-tagged TrkA-p75 and EA-tagged . Activation of TrkA-p75 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0529Y3-00148 1000 dp (10 x 96-well) $7,729.00
93-0529Y3-00147 200 dp (2 x 96-well) $2,249.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Neurotrophic tyrosine kinase receptor type 1
Group
Receptor Tyrosine Kinase
SH2 Domain
SHC1
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_002529
Agonist/Ligand
Recombinant Human b-NGF
Assay Measures
SH2 Recruitment
Bioassay Target Name
TrkA-p75
The following graph is an example of a typical dose response curve for the TrkA-p75 Bioassay generated using the protocol outlined in this manual. The data shows potent, dose-dependent SH2 recruitment, when TrkA-p75 is treated with Recombinant Human β-NGF.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® TrkA-p75 Bioassay Kit

    The PathHunter TrkA-p75 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to study drug potency and neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The cryopreserved cells have been manufactured to en...

    [User Manual] PathHunter® TrkA-p75 Bioassay Kit