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PathHunter® U2OS PDGFRa Bioassay Kit

The PathHunter U2OS PDGFRa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Recombinant Human Growth Hormone) based on activation of PDGFRa Kinase activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged PDGFRa and EA-tagged . Activation of PDGFRa stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0823Y3-00072 1000 dp (10 x 96-well) $8,975.00
93-0823Y3-00071 200 dp (2 x 96-well) $2,780.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Platelet-derived growth factor receptor alpha
Group
Receptor Tyrosine Kinase
SH2 Domain
PLCG1
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_006206.4
Agonist/Ligand
Recombinant Human PDGF-BB
Assay Measures
SH2 Recruitment
Bioassay Target Name
PDGFRa
Assay Qualified With
PDGF-BB
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A

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