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PathHunter® U2OS MCHR1 Bioassay Kit

The PathHunter U2OS MCHR1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Recombinant Human PDGF-BB) based on activation of MCHR1 GPCR activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged MCHR1 and EA-tagged . Activation of MCHR1 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0940Y3-00150 1000 dp (10 x 96-well) $8,975.00
93-0940Y3-00149 200 dp (2 x 96-well) $2,780.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Melanin concentrating hormone receptor 1
Group
Melanin-concentrating hormone
Cell Background
U2OS
Receptor Species
Human
Accession Number
AY562945
Agonist/Ligand
Melanin Concentrating Hormone (6-17)
Assay Measures
β-Arrestin
Bioassay Target Name
MCHR1
The following graph is an example of a typical dose-response curve for the MCHR1 Bioassay generated using the protocol outlined in this manual. The data shows potent, dose-dependent recruitment with β-arrestin, when treated with MCH (6-17). The plate was read on the EnVision® Multimode Plate Reader and data analysis was conducted using GraphPad Prism.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gi/Go