PathHunter® U2OS MCHR1 Bioassay Kit

The PathHunter MCHR1 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to determine drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. This bioassay has been optimized for a 96-well plate format. The protocol can be optimized further for running the assay in a high-throughput 384-well plate format.

Catalog No. Size Price Quantity
93-0940Y3-00150 1000 dp (10 x 96-well) $7,159.00
93-0940Y3-00149 200 dp (2 x 96-well) $2,069.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Melanin concentrating hormone receptor 1
Group
Melanin-concentrating hormone
Cell Background
U2OS
Species
Human
Accession Number
AY562945
Agonist/Ligand
Melanin Concentrating Hormone (6-17)
Assay Measures
β-Arrestin
Bioassay Target Name
MCHR1
The following graph is an example of a typical dose-response curve for the MCHR1 Bioassay generated using the protocol outlined in this manual. The data shows potent, dose-dependent recruitment with β-arrestin, when treated with MCH (6-17). The plate was read on the EnVision® Multimode Plate Reader and data analysis was conducted using GraphPad Prism.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gi/Go