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PathHunter® U2OS IL31RA/OSMRb Dimerization Bioassay Kit

The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.

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Related Family Members:

Target/Description
Interleukin 31 receptor alpha
Group
Interleukin Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_139017.5
Agonist/Ligand
IL-31
Bioassay Target Name
IL31RA/OSMRb
Assay Qualified With
IL-31
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® Dimerization Bioassay Kit

    PathHunter® Dimerization Bioassay Kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study receptor-receptor interactions at the cell surface. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay ...

    [User Manual] PathHunter® Dimerization Bioassay Kit