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InCELL Hunter™ eXpress U2OS Bcl2-Bim Protein Binding Assay

Discontinued: Please refer to the alternative product 96-0008C3 (InCELL Hunter™ U2OS Bcl2-Bim Protein Binding Cell Line).

InCELL Hunter assay detects degradation of a partner protein that is ordinarily stabilized by its natural interaction with the target protein. The cell lines are engineered to co-express an untagged target protein, and an Enzyme Donor (ED) tagged partner protein. Either protein may be full length or truncated. Addition of inhibitors disrupting the interaction of the two proteins results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
96-0008E3CP0S 100 dp (1 x 96-well)
96-0008E3CP0M 200 dp (2 x 96-well)
96-0008E3CP0L 1000 dp (10 x 96-well)

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Target/Description
BCL2 B-cell CLL/lymphoma 2
Group
BCL2
Cell Background
U2OS
Species
Human
Accession Number
NM_000633
Agonist/Ligand
ABT-737
Assay Measures
Intracellular Binding
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, signal was detected using the InCELL Hunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] InCELL Hunter™ eXpress Protein Binding Assays

    InCELL Hunter™ eXpress protein binding assay provides a cell-based approach to look at protein stability upon compound binding. The assay described in this booklet has been validated for use in 96-well and 384-well microplate formats. The eXpress kits contain all reagents needed for a complete experiment including cells, cell plating reagents, detection...

    [User Manual] InCELL Hunter™ eXpress Protein Binding Assays