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PathHunter® eXpress TrkA-P75 Functional Assay

PathHunter® RTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged RTK, an Enzyme Acceptor (EA) tagged SH2 domain, and, in some cases, an untagged co-receptor. Activation of the RTK-PK induces receptor dimerization, leading to SH2-EA recruitment, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0529E3CP16S 100 dp (1 x 96-well) $1378.00
93-0529E3CP16M 200 dp (2 x 96-well) $2058.00
93-0529E3CP16L 1000 dp (10 x 96-well) $7155.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Neurotrophic tyrosine kinase receptor type 1
Group
Receptor Tyrosine Kinase
SH2 Domain
SHC1
Cell Background
U2OS
Species
Human
Accession Number
NM_002529
Agonist/Ligand
Recombinant Human b-NGF
Assay Measures
Receptor Phosphorylation
Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
NA