The PathHunter Tocilizumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. IL-6) based on activation of IL6R/IL6ST Cytokine activity via detection of Dimerization. The included cell line overexpresses ProLink-tagged IL6R/IL6ST and EA-tagged . Activation of IL6R/IL6ST stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Actemra (not supplied in the bioassay kit). Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.