PathHunter® Tocilizumab Bioassay Kit

The PathHunter Tocilizumab Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. IL-6) based on activation of IL6R/IL6ST Cytokine activity via detection of Dimerization. The included cell line overexpresses ProLink-tagged IL6R/IL6ST and EA-tagged . Activation of IL6R/IL6ST stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Actemra (not supplied in the bioassay kit). Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-1045B3-00110 1000 dp (10 x 96-well) $8,259.00
93-1045B3-00109 200 dp (2 x 96-well) $2,399.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
interleukin 6 receptor
Group
Interleukin Receptor
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_000565.3
Agonist/Ligand
IL-6
Assay Measures
Dimerization
Bioassay Target Name
IL6R/IL6ST
Assay Qualified With
Actemra
Drug Trademark Owner
Actemra is a registered trademark of Chugai Seiyaku Kabushiki Kaisha Corp., a member of the Roche Group.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A