PathHunter® eXpress TIM3/TIM3 Dimerization Assay

The PathHunter TIM3/TIM3 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand induced dimerization of TIM3/TIM3 receptors. This product uses EFC technology. The cell line included overexpresses TIM3 target protein fused to PK alongside TIM3 target protein fused to EA. Upon ligand-induced dimerization of TIM3/TIM3 , detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-1082E3CP5M 200 dp (2 x 96-well) $2,279.00
93-1082E3CP5L 1000 dp (10 x 96-well) $7,479.00

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Target/Description
T-cell immunoglobulin mucin receptor 3
Group
T cell receptor (inhibitory)
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_032782.4
Agonist/Ligand
Anti-TIM3 Antibody [344801]
Assay Measures
Dimerization
Bioassay Target Name
TIM3/TIM3
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the Cell Seeding Time indicated below. Cells were then stimulated with a control compound using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A