cAMP Hunter™ Teriparatide Bioassay Kit

The cAMP Hunter Teriparatide Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Luteinizing hormone) based on activation of PTHR GPCR activity via detection of cAMP. The included cell line overexpresses ProLink-tagged PTHR and EA-tagged . Activation of PTHR stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with ‎Forteo® (not supplied in the bioassay kit). ‎Forteo® is a registered trademark of Eli Lilly and Company. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
95-0118Y2-00058 1000 dp (10 x 96-well) $8,259.00
95-0118Y2-00057 200 dp (2 x 96-well) $2,399.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Parathyroid hormone receptor 1
Group
Parathyroid hormone
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
AY449732
Agonist/Ligand
PTH(1-34)
Assay Measures
cAMP
Bioassay Target Name
PTHR1
Assay Qualified With
‎Forteo®
Drug Trademark Owner
‎Forteo® is a registered trademark of Eli Lilly and Company
The following graph is an example of a typical dose-response curve for teriparatide generated using the protocol outlined in this Teriparatide Bioassay Kit user manual. The data shows potent, dose-dependent accumulation of cAMP, when treated with teriparatide
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gs & Gq
  • [User Manual] cAMP Hunter™ Teriparatide Bioassay Kit

    cAMP Hunter Teriparatide Bioassay Kits provide a functional, robust, highly sensitive, and easy-to-use cell-based assay to study Teriparatide potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The pre-qu...

    [User Manual] cAMP Hunter™ Teriparatide Bioassay Kit