PathHunter® eXpress TBXA2R U2OS β-Arrestin GPCR Assay

PathHunter® eXpress β-Arrestin GPCR cells are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0322E3CP5M 200 dp (2 x 96-well) $2,069.00
93-0322E3CP5L 1000 dp (10 x 96-well) $7,159.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Thromboxane A2 receptor
Group
Prostanoid
Cell Background
U2OS
Species
Human
Accession Number
NM_001060
Agonist/Ligand
I-BOP
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gq