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PathHunter® eXpress TACR2 Activated GPCR Internalization Assay

PathHunter® eXpress Activated GPCR Internalization cells are engineered to co-express an untagged GPCR, Enzyme Acceptor (EA) tagged β-Arrestin, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR induces β-Arrestin recruitment, followed by internalization of the Receptor/Arrestin-EA complex in PK-tagged endosomes. This forces complementation of the two β-galactosidase enzyme fragments (EA and PK), forming functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0677E3CP0S 100 dp (1 x 96-well) $1378.00
93-0677E3CP0M 200 dp (2 x 96-well) $2058.00
93-0677E3CP0L 1000 dp (10 x 96-well) $7155.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Tachykinin receptor 2
Group
Tachykinin
Cell Background
U2OS
Species
Human
Accession Number
AY322545
Agonist/Ligand
Substance P
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gq
  • [User Manual] PathHunter® eXpress Activated GPCR Internalization Assay

    PathHunter® eXpress Activated GPCR Internalization Assays are ready to use complete kits that contain everything you need to measure arrestin bound internalized GPCRs in live cells without the hassle of using antibodies, radioactivity or elaborate imaging. The eXpress kits include single use vials of frozen cells stably expressing the GPCR of i...

    [User Manual] PathHunter® eXpress Activated GPCR Internalization Assay