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PathHunter® eXpress SYK Activity Assay

PathHunter® CTK Activity cell lines are engineered to express the catalytic domain from CTK of interest fused to the cytokine receptor binding domain (CRBD) of a JAK kinase. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink™ (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the receptor-PK. Activation of the cytokine receptor-PK induces receptor phosphorylation by the CTK catalytic domain. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0865E3CP16S 100 dp (1 x 96-well) $847.00
93-0865E3CP16M 200 dp (2 x 96-well) $1239.00
93-0865E3CP16L 1000 dp (10 x 96-well) $4938.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Growth hormone receptor
Group
Cytosolic Tyrosine Kinase
SH2 Domain
PLCG1
Cell Background
U2OS
Species
Human
Accession Number
NM_000163
Agonist/Ligand
Recombinant Human Growth Hormone
Antagonist/Inhibitor
MNS
Assay Measures
Receptor Phosphorylation
Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then pre-incubated with inhibitor, followed by stimulation with the control agonist . Following agonist stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A