PathHunter® Sargramostim Bioassay Kit

The PathHunter Sargramostim Bioassay is an application of the DiscoverX Dimerization Assay platform, which can be used to detect ligand-induced functional dimerization of two subunits of a receptor-dimer pair. This assay detects GM-CSF-induced heterodimerization of the CSF2RA and CSF2RB receptors. The cells have been stably engineered to co-express CSF2RB fused to PK, and CSF2RA fused to EA. Binding of GM-CSF to the cytokine-specific CSF2RA receptor leads to heterodimerization with CSF2RB, leading to activation of intracellular signaling pathways. Receptor dimerization forces the two enzyme fragments to complement, resulting in the formation of a functional b-gal enzyme. The enzyme hydrolyzes a substrate to generate a chemiluminescent signal. Sargramostim binds to CSF2RA, stimulating receptor heterodimerization with the CSF2RB receptor, leading to an increase in signal. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.

Catalog No. Size Price Quantity
93-1078Y3-00112 1000 dp (10 x 96-well) $8,259.00
93-1078Y3-00111 200 dp (2 x 96-well) $2,399.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
CSF2RB protein
Group
Cytokine receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_000395.2
Agonist/Ligand
GM-CSF
Assay Measures
Dimerization
Bioassay Target Name
CSF2RB/CSF2R
Assay Qualified With
Leukine®
Drug Trademark Owner
Leukine® is a registered trademark of Genzyme Corporation (licensed)
The graph is a representative dose-response curve with Sargramostim. Using the protocol outlined in this user manual, the data show a potent, dose-dependent stimulation of dimerization of the components of the GM-CSF receptor (CSFR2A and CSF2RB) in U2OS cells when treated with a dose response of Sargramostim.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A