PathHunter® Sargramostim Bioassay Kit

The PathHunter Sargramostim Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Anti-PD1 Antibody [NAT105]) based on activation of CSF2RB/CSF2RA Cytokine activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged CSF2RB/CSF2RA and EA-tagged . Activation of CSF2RB/CSF2RA stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Leukine® (not supplied in the bioassay kit). Leukine® is a registered trademark of Genzyme Corporation (licensed). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-1078Y3-00112 1000 dp (10 x 96-well) $8,259.00
93-1078Y3-00111 200 dp (2 x 96-well) $2,399.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

Related Family Members:

Target/Description
CSF2RB protein
Group
Cytokine receptor
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_000395.2
Agonist/Ligand
GM-CSF
Assay Measures
Dimerization
Bioassay Target Name
CSF2RB/CSF2R
Assay Qualified With
Leukine®
Drug Trademark Owner
Leukine® is a registered trademark of Genzyme Corporation (licensed)
The graph is a representative dose-response curve with Sargramostim. Using the protocol outlined in this user manual, the data show a potent, dose-dependent stimulation of dimerization of the components of the GM-CSF receptor (CSFR2A and CSF2RB) in U2OS cells when treated with a dose response of Sargramostim.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A