PathHunter® PTH Bioassay Kit

The PathHunter PTH Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. PTH(1-34)) based on activation of PTHR GPCR activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR and EA-tagged β-Arrestin-2. Activation of PTHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0315Y2-00048 1000 dp (10 x 96-well) $8,259.00
93-0315Y2-00047 200 dp (2 x 96-well) $2,399.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Parathyroid hormone receptor 1
Group
Parathyroid hormone
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
AY449732
Agonist/Ligand
PTH(1-34)
Assay Measures
β-Arrestin
Bioassay Target Name
PTHR1
Assay Qualified With
PTH (1-34)
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gs & Gq
  • [User Manual] PathHunter® PTH Bioassay Kit

    PathHunter® Parathyroid Hormone (PTH) Bioassay kits are ready to use kits that contain everything necessary to perform a functional assay with live cells, without any continuous cell culture. This kit has been designed to rapidly detect the potency of your biologic or the presence of neutralizing antibodies against the biologic. The Bioassay ki...

    [User Manual] PathHunter® PTH Bioassay Kit