PathHunter® eXpress PRLR-JAK2 Functional Assay

PathHunter® CTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged Cytokine receptor, an untagged CTK, and an Enzyme Acceptor (EA) tagged SH2 domain. Activation of the Receptor-PK induces phosphorylation of the receptor by the CTK. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0687E3CP0M 200 dp (2 x 96-well) $2,279.00
93-0687E3CP0L 1000 dp (10 x 96-well) $7,479.00

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Related Family Members:

Prolactin receptor
Cytosolic Tyrosine Kinase
SH2 Domain
Cell Background
Receptor Species
Accession Number
Recombinant Human Prolactin
Assay Measures
Receptor Phosphorylation
Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
See User Manual for Product Configuration Details
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
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