The PathHunter PD-1 (SHP1) Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Anti-CD47 Antibody (Ab-2; Clone B6H12.2)) based on activation of PD-1, SHP1 Checkpoint activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged PD-1 target protein and and EA-tagged SH2 domain protein. Activation of PD-1, SHP1 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.