PathHunter® eXpress P2RY1 Activated GPCR Internalization Assay

PathHunter® eXpress Activated GPCR Internalization cells are engineered to co-express an untagged GPCR, Enzyme Acceptor (EA) tagged β-Arrestin, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR induces β-Arrestin recruitment, followed by internalization of the Receptor/Arrestin-EA complex in PK-tagged endosomes. This forces complementation of the two β-galactosidase enzyme fragments (EA and PK), forming functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0967E3CP0M 200 dp (2 x 96-well) $2,069.00
93-0967E3CP0L 1000 dp (10 x 96-well) $7,159.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

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Target/Description
Purinergic receptor P2Y, G-protein coupled, 1
Group
P2Y
Cell Background
U2OS
Species
Human
Accession Number
NM_002563.2
Agonist/Ligand
2-(methylthio)-ATP
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gq