PathHunter® eXpress OPRM1 Total GPCR Internalization Assay

PathHunter® eXpress Total GPCR Internalization cells are engineered to co-express the ProLink™ (PK) tagged GPCR, and an Enzyme Acceptor (EA) tag localized to the endosomes. Activation of the GPCR-PK induces internalization of the receptor in EA-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0745E3CP5M 200 dp (2 x 96-well) $2,009.00
93-0745E3CP5L 1000 dp (10 x 96-well) $6,939.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Opioid receptor mu
Group
Opioid
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_000914.3
Agonist/Ligand
Met 5-Enkephalin
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gi/Go
  • [User Manual] PathHunter® eXpress Total GPCR Internalization Assay

    PathHunter® eXpress Total GPCR Internalization Assays are ready to use complete kits that contain everything you need to measure internalized GPCRs in live cells but without imaging, antibodies or radioactivity. The eXpress kits include single use vials of frozen cells stably expressing the GPCR of interest, optimized cell plating reagent, chemiluminescent detection reagents and ...

    [User Manual] PathHunter® eXpress Total GPCR Internalization Assay