The PathHunter Jurkat SIRPa Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. TGR5 Receptor Agonist) based on activation of SIRPa Checkpoint activity via detection of cAMP. The included cell line overexpresses ProLink-tagged SIRPa and EA-tagged . Activation of SIRPa stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.