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PathHunter® Insulin Bioassay Kit

The PathHunter Insulin Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Insulin, Human) based on activation of INSRb Kinase activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged INSRb and EA-tagged . Activation of INSRb stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with USP Insulin (not supplied in the bioassay kit). USP Insulin is a registered trademark of . Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0466Y3-00008 1000 dp (10 x 96-well) $8,999.00
93-0466Y3-00007 200 dp (2 x 96-well) $2,609.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Insulin receptor, isoform B
Group
Receptor Tyrosine Kinase
SH2 Domain
mPLCG1
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_000208
Agonist/Ligand
Insulin, Human
Assay Measures
SH2 Recruitment
Bioassay Target Name
INSRb
Assay Qualified With
USP Insulin
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A

  • [User Manual] PathHunter® Insulin Bioassay Kit

    PathHunter® Insulin Bioassay kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study insulin potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, dilution buffer, and ass...

    [User Manual] PathHunter® Insulin Bioassay Kit