The PathHunter Insulin Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Insulin, Human) based on activation of INSRb Kinase activity via detection of SH2 Recruitment. The included cell line overexpresses ProLink-tagged INSRb and EA-tagged . Activation of INSRb stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with USP Insulin (not supplied in the bioassay kit). USP Insulin is a registered trademark of . Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.