PathHunter® eXpress IL23R/IL12RB1 Dimerization Assay

The PathHunter® Dimerization assay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-1007E3CP0S 100 dp (1 x 96-well) $1378.00
93-1007E3CP0M 200 dp (2 x 96-well) $2058.00
93-1007E3CP0L 1000 dp (10 x 96-well) $7155.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Interleukin 23 receptor 
Group
Interleukin Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_144701.2
Agonist/Ligand
IL-23
Assay Measures
IL23R/IL12RB1 Dimerization
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the Cell Seeding Time indicated below. Cells were then stimulated with a control compound using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® eXpress Dimerization Assay Kit

    PathHunter® eXpress Dimerization Assay Kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study receptor-receptor interactions at the cell surface. The eXpress kits contain all the reagents needed for a complete assay  [User Manual] PathHunter® eXpress Dimerization Assay Kit