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PathHunter® eXpress IL1R1/IL1RAP Dimerization Assay

The PathHunter® Dimerization assay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-1032E3CP5S 100 dp (1 x 96-well) $1405.00
93-1032E3CP5M 200 dp (2 x 96-well) $1998.00
93-1032E3CP5L 1000 dp (10 x 96-well) $6679.00

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Target/Description
Interleukin 1 receptor, type I
Group
Interleukin Receptor
Cell Background
U2OS
Species
Human
Accession Number
NM_000877.3
Agonist/Ligand
IL-1α
Assay Measures
IL1R1/IL1RAP Dimerization
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2 for the Cell Seeding Time indicated below. Cells were then stimulated with a control compound using the assay conditions described below. Following stimulation, signal was detected using the PathHunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
2 vials, 1 x 106 cells per vial in 0.1 mL
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® eXpress Dimerization Assay Kit

    PathHunter® eXpress Dimerization Assay Kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study receptor-receptor interactions at the cell surface. The eXpress kits contain all the reagents needed for a complete assay  [User Manual] PathHunter® eXpress Dimerization Assay Kit