PathHunter® eXpress HTR2C (VNV) U2OS β-Arrestin GPCR Assay

PathHunter® eXpress β-Arrestin GPCR cells are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0938E3CP19S 100 dp (1 x 96-well) $1378.00
93-0938E3CP19M 200 dp (2 x 96-well) $2058.00
93-0938E3CP19L 1000 dp (10 x 96-well) $7155.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
5-hydroxytryptamine receptor 2C - VNV isoform
Group
5-Hydroxytryptamine
Cell Background
U2OS
Species
Human
Accession Number
NM_000868.2
Agonist/Ligand
Serotonin/5-HT
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Gq
  • [User Manual] PathHunter® β-Arrestin eXpress GPCR Assay

    PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e., β-arrestin recruitment to activated GPCRs, enabling scientist...

    [User Manual] PathHunter® β-Arrestin eXpress GPCR Assay