cAMP Hunter™ eXpress HRH2 CHO-K1 GPCR Assay

cAMP Hunter™ eXpress cells are engineered to overexpress naturally Gi or Gs coupled, wildtype GPCRs and are designed to detect changes in intracellular cAMP levels in response to agonist stimulation of the receptor. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
95-0043E2CP2M 200 dp (2 x 96-well) $1,346.00
95-0043E2CP2L 1000 dp (10 x 96-well) $4,986.00

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Target/Description
Histamine receptor H2
Group
Histamine
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
AY136744
Agonist/Ligand
Histamine
Cells were plated in a 96-well plate and stimulated with a control agonist (+ Forskolin for Gi targets). Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gq & Gs
  • [User Manual] cAMP Hunter™ eXpress GPCR Assays

    The cAMP Hunter eXpress GPCR Assay can be used for small or large molecules. The kits provide a robust, highly sensitive and easy-to-use assay for monitoring G-protein coupled receptor (GPCR) activation based on 3'-5'-cyclic adenosine monophosphate (cAMP) production in cells.

    [User Manual] cAMP Hunter™ eXpress GPCR Assays