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PathHunter® eXpress HEK 293 IκB Degradation Assay

PathHunter Degradation cells are engineered to express an Enzyme Donor (ED) tagged target protein. Activation of the pathway results in degradation of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0538E1CP7S 100 dp (1 x 96-well) $1378.00
93-0538E1CP7M 200 dp (2 x 96-well) $2058.00
93-0538E1CP7L 1000 dp (10 x 96-well) $7155.00
For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.
Target/Description
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Group
Pathway
Cell Background
HEK 293
Species
Human
Accession Number
NM_020529
Agonist/Ligand
TNFα (human)
Assay Measures
IkB Degradation
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, Signal was detected using the PathHunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A

  • [User Manual] PathHunter eXpress Degradation Assays

    PathHunter eXpress Pathway assay kits provide a robust, highly sensitive and easy-to-use method cell-based functional assay for IκB degradation and NFκB pathway activation. The eXpress kits contain everything needed for a complete IκB degradation assay including cells, detection reagents, media, and plates. The prevalidated, frozen eXpress cel...

    [User Manual] PathHunter eXpress Degradation Assays