Find a Product

InCELL Hunter™ eXpress HEK 293 BRDT(1,2) Bromodomain Assay

The InCELL Hunter technology measures compound binding to an intracellular target by detecting changes in protein stability. In this assay, the target is fused to the enhanced ProLabel®(ePL) enzyme fragment and expressed in the selected cell background. The amount of fusion protein is quantified through the addition of cell lysis buffer and a complementary enzyme acceptor fragment (EA). The two enzyme fragments complement to form a functional enzyme that is capable of hydrolyzing substrate and generating a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
96-0046E1CP7S 100 dp (1 x 96-well) $847.00
96-0046E1CP7M 200 dp (2 x 96-well) $1239.00
96-0046E1CP7L 1000 dp (10 x 96-well) $4938.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Bromodomain containing testis-specific, 1st & 2nd bromodomains
Group
Bromodomain
Cell Background
HEK 293
Species
Human
Accession Number
NM_001726
Agonist/Ligand
(+)-JQ1
Assay Measures
Intracellular Binding
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, signal was detected using the InCELL Hunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] InCELL Hunter™ eXpress Bromodomain Assays

    InCELL Hunter™ eXpress Bromodomain assays provide a cell-based approach to look at protein stability upon compound binding. The assay described in this booklet has been validated for use in 96-well and 384-well microplate formats. The eXpress kits contain all reagents needed for a complete experiment including cells, cell plating reagents, detection rea...

    [User Manual] InCELL Hunter™ eXpress Bromodomain Assays