InCELL Hunter™ eXpress HEK 293 BRD3(1,2) Bromodomain Assay

The InCELL Hunter technology measures compound binding to an intracellular target by detecting changes in protein stability. In this assay, the target is fused to the enhanced ProLabel®(ePL) enzyme fragment and expressed in the selected cell background. The amount of fusion protein is quantified through the addition of cell lysis buffer and a complementary enzyme acceptor fragment (EA). The two enzyme fragments complement to form a functional enzyme that is capable of hydrolyzing substrate and generating a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
96-0038E1CP7M 200 dp (2 x 96-well) $1,249.00
96-0038E1CP7L 1000 dp (10 x 96-well) $4,939.00

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Target/Description
bromodomain-containing protein 3, 1st & 2nd bromodomains
Group
Bromodomain
Cell Background
HEK 293
Receptor Species
Human
Accession Number
NM_007371.3
Agonist/Ligand
(+)-JQ1
Assay Measures
Intracellular Binding
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, signal was detected using the InCELL Hunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] InCELL Hunter™ eXpress Assays

    InCELL Hunter™ eXpress Assays provide a cell-based approach to evaluate changes in protein stability upon compound binding. These assay kits have been validated for use in 96-well and 384-well microplate formats. The eXpress kits contain all the materials needed for a complete assay including cells, cell plating reagents, detection reagents, and plates. The pre-validated, cryopreserved eX...

    [User Manual] InCELL Hunter™ eXpress Assays