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InCELL Hunter™ eXpress HCK Kinase Binding Assay

InCELL Hunter technology measures compound binding to an intracellular target by detecting changes in protein stability. In this assay, the target is fused to the enhanced ProLabel®(ePL) enzyme fragment and expressed in the selected cell background. The amount of fusion is quantified through the addition of substrate and complementary enzyme acceptor fragment (EA). The small size, but robust activity of the ePL tag makes it an ideal fusion partner. The small disordered peptide has minimal effects on target half-life, and generates high levels of enzyme activity, resulting in a sensitive measurement of protein levels. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
96-0041E15CP0S 100 dp (1 x 96-well) $847.00
96-0041E15CP0M 200 dp (2 x 96-well) $1239.00
96-0041E15CP0L 1000 dp (10 x 96-well) $4938.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Group
Cytosolic Tyrosine Kinase
Cell Background
A549
Antagonist/Inhibitor
Dasatinib
Assay Measures
Intracellular Binding
Cells were plated in a 96-well plate and incubated at 37°C/5% CO2. Cells were then stimulated with a control compound, using the assay conditions described below. Following stimulation, signal was detected using the InCELL Hunter Detection Reagents provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
  • [User Manual] InCELL Hunter™ eXpress Kinase Binding Assays

    InCELL Hunter™ eXpress kinase binding assays provide a cell-based approach to look at protein stability upon compound binding. The assay described in this booklet has been validated for use in 96-well and 384-well microplate formats. The eXpress kits contain all reagents needed for a complete experiment including cells, cell plating reagents, detection ...

    [User Manual] InCELL Hunter™ eXpress Kinase Binding Assays