PathHunter® eXpress GPR65 CHO-K1 β-Arrestin Orphan GPCR Assay

PathHunter® eXpress β-Arrestin Orphan GPCR cells are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0428E2ACP1S 100 dp (1 x 96-well) $1378.00
93-0428E2ACP1M 200 dp (2 x 96-well) $2058.00
93-0428E2ACP1L 1000 dp (10 x 96-well) $7155.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
G-protein coupled receptor 65
Group
Class A Orphan
Cell Background
CHO-K1
Species
Human
Accession Number
NM_003608
Agonist/Ligand
N/A
Figure 1. Cell lysates prepared from PathHunter® Orphan GPCR β-Arrestin cell lines were treated with PNGase F (Glyko: GKE-5003), run on a SDS-PAGE gel and analyzed using the PathHunter® EAstern Blot Kit (DRx: 93-0053). Untreated lane resolves a band of appropriate size corresponding to GPCR-PK fusion protein and the PNGase F treated lane resolves a deglycosylated band indicative of proper expression and folding of GPCR protein.
Figure 2. PathHunter® eXpress cells were analyzed for basal activity as well as GPCR-ProLink™ expression by comparing the ratio of signal between untreated cells and cells treated with saturating amounts of exogenous EA, using ProLink™ Detection Kit (DRx: 92-0006). Signal from complementation of ProLink™ and EA fragments correlates to the amount of GPCR-PK expression in the cell line.
Figure 3. Viability of PathHunter® eXpress cells were confirmed by bright field microscopy.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
β-Arrestin-2
Coupling
Unknown
  • [User Manual] PathHunter® β-Arrestin eXpress GPCR Assay

    PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e., β-arrestin recruitment to activated GPCRs, enabling scientist...

    [User Manual] PathHunter® β-Arrestin eXpress GPCR Assay