PathHunter® GPCR Explorer Kit

This product has been discontinued. Please contact Technical Support for more information regarding custom development or alternate products.

PathHunter β-arrestin1 and 2 EA parental cell lines allow for introduction of your own GPCRs (or other β-arrestin binding protein) and development of your own β-arrestin recruitment assays. PathHunter β-arrestin assays take advantage of DiscoverX’s proprietary enzyme fragment complementation technology. The GPCR (or other β-arrestin binding protein) is fused in frame with a small enzyme donor fragment of β-galactosidase (β-gal) called ProLink™ (PK) which is then co-expressed in the EA parental cell line stably expressing a fusion protein of β-arrestin with a larger fragment of β-gal called enzyme acceptor (EA). Activation of the GPCR-PK (or other PK-tagged β-arrestin binding protein), which induces binding to the β-arrestin-EA fusion protein, forces complementation of the two β-gal enzyme fragments, resulting in the formation of an active β-gal enzyme. Addition of PathHunter detection reagents results in generation of a chemiluminescent signal, allowing the interaction of β-arrestin with GPCR or β-arrestin binding protein to be detected.

Catalog No. Size Price Quantity
93-0164E2V2L 1000 dp (10 x 96-well)

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Cell Background
Recombinant Human MIP-1α (CCL3)
See User Manual for Product Configuration Details
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
β-Arrestin Isoform
  • [User Manual] PathHunter® GPCR Explorer Kit

    PathHunter® GPCR Explorer kits are a simple and easy-to-use method for building on-demand functional GPCR assays. Whether you have a panel of mutant GPCR cDNAs, a favorite ortholog GPCR or a GPCR heterodimer you’d like to funct...

    [User Manual] PathHunter® GPCR Explorer Kit