PathHunter® eXpress GCGR Total GPCR Internalization Assay

PathHunter® eXpress Total GPCR Internalization cells are engineered to co-express the Enzyme Acceptor (EA) tagged GPCR, and a ProLink™ (PK) tag localized to the endosomes. Activation of the GPCR-EA induces internalization of the receptor in PK-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0820E1CP0M 200 dp (2 x 96-well) $2,279.00
93-0820E1CP0L 1000 dp (10 x 96-well) $7,479.00

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Target/Description
Glucagon receptor
Group
Glucagon
Cell Background
HEK 293
Receptor Species
Human
Accession Number
NM_000160
Agonist/Ligand
Glucagon
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gs
  • [User Manual] PathHunter® eXpress Total GPCR Internalization Assay

    PathHunter® eXpress Total GPCR Internalization Assays are ready to use complete kits that contain everything you need to measure internalized GPCRs in live cells but without imaging, antibodies or radioactivity. The eXpress kits include single use vials of frozen cells stably expressing the GPCR of interest, optimized cell plating reagent, chemiluminescent detection reagents and ...

    [User Manual] PathHunter® eXpress Total GPCR Internalization Assay