The cAMP Hunter Follitropin Alfa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. PTH(1-34)) based on activation of FSHR GPCR activity via detection of cAMP. The included cell line overexpresses ProLink-tagged FSHR and EA-tagged . Activation of FSHR stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Gonal-F (not supplied in the bioassay kit). Gonal-F is a registered trademark of Merck KGAA. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.