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PathHunter® Epoetin Alfa Bioassay Kit

The PathHunter Epoetin Alfa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. CP 55,940) based on activation of EPOR Kinase activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged EPOR and EA-tagged . Activation of EPOR stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Aranesp® (not supplied in the bioassay kit). Aranesp® is a registered trademark of Amgen Inc.. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
93-0965Y3-00018 1000 dp (10 x 96-well) $8,259.00
93-0965Y3-00017 200 dp (2 x 96-well) $2,399.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Erythropoietin receptor
Group
Cytokine Receptor
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_000121.3
Agonist/Ligand
Erythropoietin
Assay Measures
Dimerization
Bioassay Target Name
EpoR-EpoR
Assay Qualified With
Recombinant hEpo
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A

  • [User Manual] PathHunter® Epoetin Alfa Bioassay Kit

    PathHunter® Epoetin Alfa Bioassay kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study epoetin alfa potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, an...

    [User Manual] PathHunter® Epoetin Alfa Bioassay Kit