The PathHunter Epoetin Alfa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. CP 55,940) based on activation of EPOR Kinase activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged EPOR and EA-tagged . Activation of EPOR stimulates the recruitment of and produces EFC signal.This assay has been optimized and qualified with Aranesp® (not supplied in the bioassay kit). Aranesp® is a registered trademark of Amgen Inc.. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.