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PathHunter® Darbepoetin Alfa Bioassay Kit

The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair. The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA). Cytoplasmic tail may have been deleted from one or both receptors. Binding of an agonist to one receptor subunit induces it to interact with its dimer partner, forcing complementation of the two enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0965Y3-00020 1000 dp (10 x 96-well) $8251.00
93-0965Y3-00019 200 dp (2 x 96-well) $2352.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Erythropoietin receptor
Group
Cytosolic Tyrosine Kinase
Cell Background
U2OS
Species
Human
Accession Number
NM_000121.3
Agonist/Ligand
Erythropoietin
Assay Measures
Dimerization
Bioassay Target Name
EpoR-EpoR
Assay Qualified With
Aranesp®
Drug Trademark Owner
Aranesp® is a registered trademark of Amgen Inc.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® Darbepoetin Alfa Bioassay Kit

    PathHunter® Darbepoetin Alfa Bioassay kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay to study darbepoetin alfa potency and neutralizing antibodies. The bioassay kits contain all the reagents needed for a complete assay including cells, detection reagents, cell plating reagent, positive control agonist, and ...

    [User Manual] PathHunter® Darbepoetin Alfa Bioassay Kit