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PathHunter® eXpress CSF3R-JAK1 Functional Assay

PathHunter® CTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged Cytokine receptor, an untagged CTK, and an Enzyme Acceptor (EA) tagged SH2 domain. Activation of the Receptor-PK induces phosphorylation of the receptor by the CTK. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0809E3CP16S 100 dp (1 x 96-well) $1405.00
93-0809E3CP16M 200 dp (2 x 96-well) $1998.00
93-0809E3CP16L 1000 dp (10 x 96-well) $6679.00

For account specific pricing, please contact Customer Service at info@discoverx.com.

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Target/Description
colony stimulating factor 3 receptor
Group
Cytosolic Tyrosine Kinase
SH2 Domain
SHC1
Cell Background
U2OS
Species
Human
Accession Number
NM_000760
Agonist/Ligand
G-CSF (Human)
Assay Measures
Receptor Phosphorylation
Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
2 vials, 1 x 106 cells per vial in 0.1 mL
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
  • [User Manual] PathHunter® eXpress Cytosolic Tyrosine Kinase Functional Assay Kit

    PathHunter® eXpress Cytosolic Tyrosine Kinase (CTK) Functional Assay kits provide a robust, highly sensitive and easy-to-use, cell-based functional assay for monitoring cytokine receptor activation or cytosolic tyrosine kinase inhibition. The eXpress kits contain everything needed for a complete CTK experiment including cells, detection reagent...

    [User Manual] PathHunter® eXpress Cytosolic Tyrosine Kinase Functional Assay Kit