PathHunter® eXpress cINSRa Functional Assay

PathHunter® RTK Functional cell lines are engineered to co-express a ProLink™ (PK) tagged RTK, an Enzyme Acceptor (EA) tagged SH2 domain, and, in some cases, an untagged co-receptor. Activation of the RTK-PK induces receptor dimerization, leading to SH2-EA recruitment, and forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-1016E3CP16M 200 dp (2 x 96-well) $2,129.00
93-1016E3CP16L 1000 dp (10 x 96-well) $7,359.00

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Related Family Members:

Target/Description
Insulin receptor, isoform A
Group
Receptor Tyrosine Kinase
SH2 Domain
PLCG1
Cell Background
U2OS
Receptor Species
Cyno
Accession Number
XM_005587742.1
Agonist/Ligand
Insulin, Human
Assay Measures
Receptor Phosphorylation
Bioassay Target Name
cINSRa
Cells were plated in a 96-well plate and incubated for 24 or 48 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A