PathHunter® eXpress CHRM4 Total GPCR Internalization Assay

PathHunter® eXpress Total GPCR Internalization cells are engineered to co-express the ProLink™ (PK) tagged GPCR, and an Enzyme Acceptor (EA) tag localized to the endosomes. Activation of the GPCR-PK induces internalization of the receptor in EA-tagged endosomes, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0902E3CP0M 200 dp (2 x 96-well) $2,009.00
93-0902E3CP0L 1000 dp (10 x 96-well) $6,939.00

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Target/Description
Cholinergic receptor, muscarinic 4
Group
Acetylcholine
Cell Background
U2OS
Receptor Species
Human
Accession Number
NM_000741.2
Agonist/Ligand
Acetylcholine
Cells were plated in a 96-well plate and stimulated with a control agonist. Following stimulation, signal was detected according to the recommended protocol.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gs & Gi/Go
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