The PathHunter CHO-K1 MRGPRX2 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Cortistatin 14) based on activation of MRGPRX2 GPCR activity via detection of β-Arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRX2 and EA-tagged . Activation of MRGPRX2 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.