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cAMP Hunter™ CHO-K1 LHCGR Bioassay Kit

The cAMP Hunter CHO-K1 LHCGR Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Peptide YY, Human) based on activation of LHCGR GPCR activity via detection of cAMP. The included cell line overexpresses ProLink-tagged LHCGR and EA-tagged . Activation of LHCGR stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
95-0106Y2-00138 1000 dp (10 x 96-well) $8,975.00
95-0106Y2-00137 200 dp (2 x 96-well) $2,780.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
Choriogonadotropin receptor
Group
Glycoprotein hormone receptor
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
NM_000233
Agonist/Ligand
Luteinizing hormone
Assay Measures
cAMP
Bioassay Target Name
LHCGR
Assay Qualified With
Human Luteinizing Hormone
The following graph is an example of a typical dose-response curve for the LHCGR Bioassay generated using the protocol outlined in the user manual. The data shows a potent, dose-dependent increase in cAMP production when treated with Human Luteinizing Hormone.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gs