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PathHunter® eXpress CHO-K1 GR Nuclear Translocation Assay

The PathHunter® eXpress Nuclear Translocation assay format detects translocation of target NHRs into the nucleus using Enzyme Fragment Complementation. The cells have been engineered to express two complementary fragments of β-galactosidase that are localized to different cellular compartments: the smaller ProLabel® (PL) fragment is fused to the NHR and localized in the cytoplasm; the larger enzyme acceptor (EA) fragment is localized in the nucleus. Activation of the NHR induces receptor translocation to the nucleus, forcing complementation of the PL and EA fragments. Activity of the resulting fully formed β-galactosidase enzyme is detected using a chemiluminescent substrate. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0002H2CP13S 48-well $451.00
93-0002H2CP13M 96-well $643.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Glucocorticoid Receptor
Group
NHR
Cell Background
CHO-K1
Species
Human
Accession Number
NM_000176
Agonist/Ligand
Dexamethasone
Assay Measures
Translocation
Cells were plated in a 48-well or 96-well plate and incubated for 24 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
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