cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit

The cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to monitor 3’-5’-cyclic adenosine monophosphate (cAMP) production in cells, as a result of ligand-mediated GPCR activation. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a high-throughput 384-well format.

Catalog No. Size Price Quantity
95-0049Y2-00140 1000 dp (10 x 96-well) $7,729.00
95-0049Y2-00139 200 dp (2 x 96-well) $2,249.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
G protein-coupled bile acid receptor 1
Group
Bile acid
Cell Background
CHO-K1
Species
Human
Accession Number
NM_001077194.1
Agonist/Ligand
TGR5 Receptor Agonist
Assay Measures
cAMP
Bioassay Target Name
GPBAR1
Assay Qualified With
TGR5 Receptor Agonist
The graph is an example of a typical dose-response curve for the GPBAR1 Bioassay generated using the protocol outlined in the manual. The data shows potent, dose-dependent cAMP production when treated with TGR5 Receptor Agonist.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gs