The cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to monitor 3’-5’-cyclic adenosine monophosphate (cAMP) production in cells, as a result of ligand-mediated GPCR activation. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a high-throughput 384-well format.