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cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit

The cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Exendin-4) based on activation of GPBAR1 GPCR activity via detection of cAMP. The included cell line overexpresses ProLink-tagged GPBAR1 and EA-tagged . Activation of GPBAR1 stimulates the recruitment of and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Catalog No. Size Price Quantity
95-0049Y2-00140 1000 dp (10 x 96-well) $8,975.00
95-0049Y2-00139 200 dp (2 x 96-well) $2,780.00
Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.

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Target/Description
G protein-coupled bile acid receptor 1
Group
Bile acid
Cell Background
CHO-K1
Receptor Species
Human
Accession Number
NM_001077194.1
Agonist/Ligand
TGR5 Receptor Agonist
Assay Measures
cAMP
Bioassay Target Name
GPBAR1
Assay Qualified With
TGR5 Receptor Agonist
The graph is an example of a typical dose-response curve for the GPBAR1 Bioassay generated using the protocol outlined in the manual. The data shows potent, dose-dependent cAMP production when treated with TGR5 Receptor Agonist.
Format
See User Manual for Product Configuration Details. The purpose of the control ligand included in the Bioassay Kit is for verification of assay performance relative to the data shown in the Certificate of Analysis for that kit. This control ligand is not intended for use as a reference standard for validation studies and lot release applications. Typically, a reference medicinal product (RMP) that has been pre-defined by the customer as an internal reference standard is used for those applications. The RMP is not provided with the bioassay kit or supplied by Eurofins DiscoverX.
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
Gs