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PathHunter® eXpress CHO-K1 ERRα Protein Interaction Assay

Discontinued: No alternative product. Please consider custom assay development for this target.

PathHunter® eXpress NHR Protein Interaction assays monitor the interaction of activated NHRs with Steroid Receptor Co-activator Peptides (SRCPs) using Enzyme Fragment Complementation. The cells are engineered to express two complementary fragments of β-galactosidase: the smaller ProLink™ (PK) fragment is fused to the NHR target of interest, and the larger Enzyme Acceptor (EA) is fused to the SRCP. Activation of the NHR target induces interaction of the receptor with the SRCP, forcing complementation of PK and EA. Activity of the resulting fully formed β-galactosidase enzyme is detected using a chemiluminescent substrate. The cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.

Catalog No. Size Price Quantity
93-0328H2CP13S 48-well
93-0328H2CP13M 96-well

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Target/Description
Estrogen related receptor alpha
Group
NHR
Cell Background
CHO-K1
Species
Human
Accession Number
NM_004451
Agonist/Ligand
XCT790
Assay Measures
Protein Interaction
Cells were plated in a 48-well or 96-well plate and incubated for 24 hours at 37°C/5% CO2. Cells were then stimulated with a control agonist. Following stimulation, signal was detected using the PathHunter® detection reagent provided in the kit according to the recommended protocol. For a detailed protocol, please refer to the user manual.
Format
See User Manual for Product Configuration Details
Storage
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Coupling
N/A
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